Does the endothelium also absorb trypan blue?

For cataract surgery trypan blue selectively stains the lens capsule and non-living cells. Healthy corneal endothelial cells are not stained in vivo, as the cellular membrane naturally prevents dye absorption from the posterior surface.

Corneal surgical techniques, such as DSEK, are different and the donor tissue can be stained with dye through in vitro application via the anterior surface of the membrane. CAPSULBlue is not currently indicated for use in DSEK surgery.